Sandwich ELISA kit for quantitative detection of sheep Luteinizing Hormone in samples from Blood, serum, plasma and other biological fluids.
Assay range: 0.05IU/ml→20IU/ml
Sensitivity: 0.027IU/ml
ASSAY PROCEDURE SUMMARY:
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at
37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10 minutes.
6. Calculate.